A faint albumin band is detected near the anode top. Pdf the reliable detection of paraprotein in serum and urine is the primary purpose of. Monoclonal free light chains flcs frequently cause rapidly progressive renal failure in patients with multiple myeloma. The immunofixation blood test is used to identify proteins called immunoglobulins in blood. Hydragel if penta immunofixation serum assay sebia. Hydragel if kits k960669 serum july 12, 1996 capillarys immunotyping k042939 serum june 27, 2005 capillarys immunotyping k082085 urine apri17, 2009 itif control k101863 serum urine november 7, 2011 paragon cze 2000 ifes control k002799 serumurine october 5, 2000. A prominent monoclonal spike mspike is detected on the gel and is visible on the corresponding electropherogram. Ife testing was performed using equipment hydrasys and reagents hydragel if. Identification of m protein from filter paper using serum protein.
Depending on the type of monoclonal protein, the lower limit of detection for this assay ranges from 12 to 25. Immunofixation electrophoresis is a simple technique that allows a protein to be. This test separates and measures proteins in your urine. Immunofixation blood test results explained the immunofixation blood test is used as a way to identify the presence of abnormal proteins. Protein and immunofixation electrophoresis lab tests online au. The process of this test, which is called electrophoresis, is also used to determine if there are normal proteins that should be present, but are not. Spe was performed on a commercial hydragel kit with a sebia. A new hydragel kit for the hydrasys agarose gel electrophoresis system is now available from sebia uk. Screening immunofixation electrophoresis sife employs a single.
Comparison between immunofixation and electrophoresis. The hydragel bence jones, when used on the hydrasys instrument, separates and characterizes bence jones proteins monoclonal free light chains or any other full monoclonal immunoglobulin, in. It looks for an abnormal protein called monoclonal protein, also called mprotein. Immunofixation has also been used to study changes in protein structure for example, glucose6phosphate dehydrogenase, and in the genetic typing of alpha1 antitrypsin. The distances that individual proteins travel depend on their size, shape and electrical charge. Proteins were stained with amido black, and densitometric scanning was performed at 570 nm hyrys densitometer, sebia. Pdf serum protein electrophoresis and immunofixation by.
Immunofixation detects monoclonal proteins in serum and urine. The kits are used in conjunction with the semiautomated hydrasys electrophoresis apparatus. Joseph mercy hospital, ann arbor, mi clinical professor of pathology, the university of michigan medical school, ann arbor, mi hodder arnold a member of the hodder headline group. The semiautomated hydrasys instrument performs migration, immunofixation and staining of the. Hydragel if predicate device k960669 intended use immunoprecipitates are stained either the hydrashift 24 daratumumab test is intended for the qualitative detection of monoclonal proteins in human serum by immunofixation electrophoresis. H5vwm kits were provided by sebia for this evaluation.
Dec 01, 2011 a, image of the patients urine pep result. Panel a is a representative example of a 54 lane sebia hydragel. Sebia hydragel if kit using the hydrasys instrument. Jul 29, 2019 immunofixation of the urine and serum should be included in a battery of tests after 23 months following diagnosis of smoldering myeloma, then every 46 months for 1 year, and, finally, every 6 12 months if the results are stable. Pdf l immunofixation est actuellement tres utilisee dans les laboratoires danalyses medicales. Depending on the type of monoclonal protein, the lower limit of detection for this assay ranges from 12 to 25 mgdl. Hydragel double if k20 2018 12 masque standard standard mask 8. In his point paper, pretorius 12 puts the case to replace spep and upep. Immunofixation by electrophoresis urine does this test have other names. The hydragel 6 if penta and hydragel 12 if penta kits are designed for the direct detection of large majority of monoclonal proteins in human serum by.
Comparison between immunofixation and electrophoresis for the. Pdf our objective was to evaluate a gel system that uses unconcentrated urine specimens for protein electrophoresis pel and immunofixation. Immunofixation electrophoresis, blood and urine definition. Serum protein electrophoresis is the easiest means of excluding the presence of a monoclonal protein and can detect bands of 0. A normal or negative result means no monclonal immunglobulins are seen in the blood sample. Immunofixation fixes certain proteins into place with antibodies and then washes away the others before staining them. Pdf serum protein electrophoresis and immunofixation by a. Protein electrophoresis and immunofixation electrophoresis detect and measure abnormal proteins, or the absence of normal proteins in blood, urine or csf.
The hydragel bence jones, when used on the hydrasys instrument, separates and characterizes bence jones proteins monoclonal free light chains or any other full monoclonal immunoglobulin, in human urine or serum. It also allows the characterization of the proteinuria. The kits are to be used in conjunction with the hydragel if kits and the semiautomated. Sebia is pleased to offer text books specifically related to serum and urine protein electrophoresis and immunofixation testing, as well as the diagnosis of hemoglobinopathies with capillary electrophoresis technology. Peaks and valleys in the bands may mean that you may be making too many or too few of certain proteins. The combination of spe and sif techniques increases up to 97% sensitivity in the detection of mprotein in patients with mm 12, 20. Agarose gels preparation agarose gels are ready to use. The hydragel 5 vwf multimers kit h5vwm was used with the hydrasys 2 scan instrumentation to perform agarose gel electrophoresis using preformed 2% agarose gels, direct immunofixation, visualization with peroxidaselabelled antibody, and densitometry.
Pdf urine protein electrophoresis and immunoelectrophoresis. Immunofixation as immunoelectrophoresis, takes place in two steps. This assay was performed as stated in the manufacturers product insert. Immunofixation electrophoresis ife has largely replaced immunoelectrophoresis iep for establishing the presence and isotype of a monoclonal immunoglobulin mprotein in serum and urine fig. The hydragel if assay is based on the principle of agarose gel electrophoresis followed by immunofixation. Common use to identify the individual types of immunoglobulins, toward diagnosing diseases such as multiple myeloma, and to evaluate effectiveness of chemotherapy. The hydragel 2 urine profile and hydragel 4 urine profile dynamic mask kits are designed for screening of main urinary proteins to type proteinuria tubular, glomerular, or mixed and or qualitative detection of free light chains and immunoglobulins g, a or m in human urine or serum, by immunofixation electrophoresis on agarose gels with specific antisera. Too much of the same immunoglobulin is usually due to different types of blood cancer. Immunofixation blood health encyclopedia university of. Protein electrophoresis in clinical diagnosis david f keren medical director, warde medical laboratory, ann arbor, mi department of pathology, st.
Micropipettor, either manual or automated, such as hydraplus sebia, pn. The immunofixation procedure on the hydrasys automate consisted of three steps. Immunoglobulins are antibodies that help your body fight infection. Linterpretation des resultats est en general facile. Immunofixation electrophoresis, blood and urine synonymacronym.
The procedure usually includes antisera to the heavy. However, in certain circumstances you may be asked to fast for 10 to 12 hours before the test. Text books solely focused on the discipline of electrophoresis testing can be difficult to find. Pdf screening immunofixation should replace protein. The hydragel if kit allows the separation and characterization of immunoglobulins heavy and light chains using specific antisera. Materials and methods electrophoretic methods semiautomated agarose electrophoresis and immunofix ation was performed with the hydrasys automate according to the manufacturers instructions, using hydragel 15 and 30 proteine and hydragel 2 and 4 immunofixation gels sebia. Use support medium for protein electrophoresis and immunofixation. During the test, an electric current is used to move the proteins across a thin layer of agarose gel. Protein electrophoresis is a method for separating the proteins found in blood serum or urine. Protein immunofixation is a wellestablished technique for typing any suspected monoclonal component on urine electrophoresis. Sebia hydragel if, hydragel if penta, hydragel bence jones standard mask and dynamic mask performed using.
The semiautomated hydrasys instrument performs migration. Immunofixation ife with use of specific antisera allows detection of the type of mc. Immunofixation was also performed using the sebia hydrasys system according to the manufactur. Pdf semiautomated agarose electrophoresis and immunofixation performed with hydrasyshyrys. Sebia, inc karen anderson director of technical and. Serum protein electrophoresis and immunofixation by a. Serum protein electrophoresis and immunofixation by a semiautomated electrophoresis system article pdf available in clinical chemistry 445.
Spe is also a method for the quantification of mc and monitoring of disease that is essential for clinical evaluation and followup of patients with plasma cell disorders. This information provides clues that a disease is affecting protein production or loss of protein. In the first step, the sample was applied by use of a template to 6 different positions on an agarose gel hydragel 2 and 4 immunofixation, sebia, and electrophoretic separation 20 w and 20 c for 9 min was done automatically. Visual examination of electrophoretic patterns by a pathologist is the most sensitive method of detecting monoclonal proteins since small bands may be obscured in densitometric scans. The high resolution immunofixation technique detects and identifies monoclonal proteins and bence jones monoclonal kappa or lambda free light chains in serum and urine to aid diagnosis and monitoring of disease. An mprotein is a type of abnormal immunoglobulin made by plasma cells. Each agarose gel in the hydragel 6 if penta and hydragel 12 if penta kits. Performance of the sebia capillarys 2 for detection and. Serum free light chain measurement aids the diagnosis of. Protein immunofixation is a wellestablished technique for the characterization of any suspected monoclonal component on serum or urine electrophoresis.
After the separation of serum proteins according to their charge, the gel is incubated with different specific antisera targeted against. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Specimen serum 1 ml collected in a gold, red, or redgraytop tube. The hydragel urine profile kit is used for proteinuria investigation by immunofixation on agarose gel in order to. On a computer screen, these proteins form a pattern of bands. Immunoassays which provide quantitative measurement of flcs in serum, have now been adopted into screening algorithms for multiple myeloma and other lymphoproliferative disorders. Immunofixationdescriptionoftheautomatedsteps incubationat20cfor5minutescontrolledbypeltiereffect. Hydragel if kits k960669 serum july 12, 1996 capillarys immunotyping k042939 serum june 27, 2005.
184 1017 632 634 133 1033 937 1547 1490 803 557 396 416 1281 484 345 1565 120 1402 300 789 743 1394 1472 1460 502 347 617 1365 604 54 625 381 1568 52 44 1132 175 237 1224 284 532 1214 504 1006